Apoptosis of human colon carcinoma HT-29 cells induced by ceramide

Table 1 Primer sequences of Bcl-2 family gene members

Gene	Primer sequence	Size (bp)	Temperature (°C)
Bax	Sense: 5’-CCAGCTGCCTTGGACTGT-3’	135	61
	Antisense: 5’-ACCCCCTCAAGACCACTCTT-3’
Bcl-xl	Sense: 5’-GTAAACTGGGGTCGCATTGT-3’	146	60
	Antisense: 5’-TGGATCCAAGGCTCTAGGTG-3’
Bcl-2	Sense: 5’-GGCAATGTGACTTTTTCCAA-3’	137	55
	Antisense: 5’-GGCTGATATTCTGCAACACTG-3’
Bad	Sense: 5’-AGGGCTGACCCAGATTCC-3’	178	60
	Antisense: 5’-GTGACGCAACGGTTAAACCT-3’
Bid	Sense: 5’-GCTTCCAGTGTAGACGGAGC-3’	116	60
	Antisense: 5’-GTGCAGATTCATGTGTGGATG-3’
β-actin	Sense: 5’-GTGGACATCCGCAAAGAC-3’	303	58
	Antisense: 5’-TCAACGCAATGTGGGAAG-3’