Effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells

Abstract

AIM: To observe the effect of solanine on the membrane potential of mitochondria in HepG₂ cells and [Ca²⁺]_i in the cells, and to uncover the mechanism by which solanine induces apoptosis.

METHODS: HepG₂ cells were double stained with AO/EB, and morphological changes of the cells were observed using laser confocal scanning microscopy (LCSM). HepG₂ cells were stained with TMRE, and change in the membrane potential of mitochondria in the cells were observed using LCSM. HepG₂ cells were double stained with Fluo-3/AM, and change of [Ca²⁺]_i in the cells were observed using LCSM. HepG₂ cells were double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca²⁺]_i in the cells were observed using LCSM.

RESULTS: Cells in treated groups showed typical signs of apoptosis. Staining with TMRE showed that solanine could lower membrane potential; staining with Fluo-3/AM showed that solanine could increase the concentration of Ca²⁺ in tumor cells; and those of double staining with TMRE and Fluo-3/AM showed that solanine could increase the concentration of Ca²⁺ in the cells at the same time as it lowered the membrane potential of mitochondria.

CONCLUSION: Solanine opens up the PT channels in the membrane by lowering the membrane po-tential, leading to Ca²⁺ being transported down its concentration gradient, which in turn leads to the rise of the concentration of Ca²⁺ in the cell, turning on the mechanism for apoptosis.

Key Words: Solanine; Hepatocarcinomatic cell; Ca²⁺ in the cell; Membrane potential; Laser confocal scanning microscopy

INTRODUCTION

Solanine is found mainly in the tuber of the potato (Solanum tuberosum L.)[1-5] and in the whole plant of the nightshade (Solanum nigrum Linn) of the family Solanaceae. The content of this substance is rather high in the green peel and the sprouts of potato and is the main toxic substance[6]. The whole plant of the nightshade contains many steroid alkaloids, including solamargine, solasonine, and solanine, as well as saponin and other substances. It can be used for anti-tumor purposes, with a strong inhibitory effect on tumors in animals and a clearly toxic effect on tumor cells[7]. Its ethanol extract is capable of inhibiting the growth of breast cancer and induce apoptosis in tumor cells[8]. The extract from the nightshade also has a strong anti-inflammatory effect because it can facilitate the formation of antibodies[9]. The anti-tumor effect of solamargine has been reported[10], but there is as yet no report about any anti-tumor effect of solanine. From our past experience in both in vivo and in vitro experiments, we have found that solanine is cytotoxic to cells, especially for the hepatocarcinomatic cell HepG₂. Through morphological observation, as well as DNA ladder and flow cytometry, we discovered that solanine exerted its anti-tumor effect by inducing apoptosis in HepG₂.

The concept "apoptosis" was officially proposed by Kerr in 1972[11]. In the three decades since it was proposed, it has always been the focus of biological researches. Especially since 1990s, the study of apoptosis has suddenly been propelled to the frontier of life science, leading to an upsurge of research activities involving almost all the fields of biomedical studies. As a result, a great deal of literature accumulated in a few years about the morphological characteristics of apoptosis, biochemical changes in apoptotic cells, genes regulating apoptosis, and various factors that can lead to apoptosis, as well as the relationship between apoptosis and health conditions. Membrane potential of mitochondria[12,13] and [Ca²⁺]_i in the cells[14,15], two factors closely associated with apoptosis, are themselves supplementary to and closely related with each other.

Rapid and continuous increase in the concentration of calcium ion is the earliest detectable biochemical change in cells undergoing apoptosis. The fact that the use of calcium-free culture medium and other methods capable of inhibiting the rise in the concentration of Ca²⁺ in cells can help to inhibit apoptosis[16] also shows that the rise in the concentration of Ca²⁺ in cells is an important condition for apoptosis to take place. A typical sign of apoptosis is the appearance of apoptotic bodies, but it is only when the concentration of Ca²⁺ has been raised that endogenous nuclease is activated, cutting the DNA chain between nucleosomes into discontinuous fragments (or apoptotic bodies), the basic unit of which is 180-200 bp long.

The mitochondrion is the storehouse for calcium in the cell, and change in its membrane potential can lead to Ca²⁺ and other ions being released from the mitochondrion or flowing into it, and the release and influx of Ca²⁺ and other ions are also the direct causes for changes in the membrane potential of mitochondrion. The essence of membrane potential change is the opening of the PT channels in the membrane. The opening of these channels precedes the release of Ca²⁺, which is followed by changes in the membrane potential.

There are many approaches to studying apoptosis. In the present study, we tried to explicate the mechanism by which solanine induced apoptosis of tumor cells by focusing on the mitochondria and by observing the effect of solanine on the membrane potential of HepG₂ and [Ca²⁺]_i in the cell (Figure 1).

Figure 1 Molecular structure of solanine.

MATERIALS AND METHODS

Materials

The cell line: HepG₂ human hepatocarcinomatic cell lines were purchased from the Institute for Cancer Research, Heilongjiang Cancer Hospital.

Test drugs: Solanine with a purity of 99% was provided by Heilongjiang Institute For Drug Control.

Chemical reagents: RPMI 1640 culture medium was supplied from HyClone Laboratories; fetal bovine serum from Hangzhou Sijiqing Biological Engineering Materials Co.,Ltd; pancreatin from Gibio; ethidium bromide (EB) from AMRESCO; acridine orange (AO) from AMRESCO; Fluo-3/AM from Biotium, US; TMRE(molecular probe) from Biotium, US; HEPES from AMRESCO.

Preparation of the reagents: Fluo-3/AM 0.1 mg/stick was diluted to suitable concentration with DMSO, and then separately loaded and stored in the dark. TMRE 25 mg/stick was made into 1 mg/mL mother solution using anhydrous alcohol, separately loaded and stored in the dark. These were diluted with buffer solution for laboratory use and added to achieve the needed final concentrations before they were used in experiments.

Apparatuses: CO₂ incubator (CO-150, NBS, US); inverted microscope (CKX-41-32, Olympus, Japan); superclean bench (SW-CJ-2F, Suzhou Purification Equipments of the Sujing Group, Suzhou); laser confocal scanning microscope (SP2, Leica, Germany) were used.

Methods

Cell culture and treatment: HepG₂ cell culture was incubated in RPMI 1640 medium containing 10% fetal bovine serum at 50 mL/L CO₂ and 37°C, and transfer of culture was performed once every 3-4 d. When the cells grew steadily in the phase of logarithmic growth, 0.25% pancreatin was used to digest the cells. Digested cells were re-suspended using RPMI 1640 medium containing 10% fetal bovine serum and counted, and the concentration of cells was adjusted to 1 × 10⁴ /mL. Cell suspension was added to the round troughs of 35 mm Petri dishes at 200 μL/dish, and the dishes were divided into 2, 0.4, 0.08, 0.016, and 0.0032 μg/mL treatment groups, the control, and the positive control (to be treated with camptothecine). After being incubated for 24 h at 50 mL/L CO₂ and 37°C, the treatment groups were treated with solanine of different concentrations so that their final concentrations were 2, 0.4, 0.08, 0.016, and 0.0032 μg/mL, respectively; an equal volume of RPMI 1640 was added to the control, while the positive control was treated with camptothecine, with a final concentration of 0.08 μg/mL. The dishes were then incubated for another 48 h at 50 mL/L CO₂ and 37°C in CO₂ incubator.

Observation of Solanine-induced morphological changes of HepG₂ nuclei using LCSM: After 48 h, the cells were taken out of the incubator. The culture solution in the Petri dishes was sucked out, and the cells were rinsed 3 times with PBS at 5 min/time, double stained with 200 μL of acridine orange (AO)/ethidium brumide (EB) so that the final concentration was 5 μg/mL, incubated for 5-10 min at 37°C, and rinsed 3 times with PBS, 5 min/time. Then 200 μL of PBS was added, and LCSM was used to observe the morphology of the cells. Dual-channel activation was used, with an excitation wavelength of 488 nm and emission wavelength of 500-520 nm for PMT1(AO) and an excitation wavelength of 543 nm and emission wavelength of 600-700 nm for PMT2(EB)[17]. Other devices included Objective APO CS40 × /1.25 oil, zoom > 1, pinhole 1.5 Airy, mode XYZ, format 512 × 512.

Observation of Solanine-induced change in [Ca²⁺]_i in HepG₂ cells using LCSM: After 48 h, the cells were taken out of the incubator. The culture solution in the Petri dishes was sucked out, and the cells were rinsed 3 times with HEPES, and 4 μg/mL Fluo-3/AM (Molecular Probes) was added, 200 μL/dish. After the cells were incubated at 37°C for 50 min and rinsed with HEPES for 3 times, 200 μL of HEPES culture medium was used to cover all the cells in the troughs, and then LCSM was used to observe the fluorescence intensity (FI) of the cells, with an excitation wavelength of 488 nm and emission wavelength of 555 ± 15 nm. Other devices included objective APO CS40 ×/1.25 oil, zoom > 1, pinhole 1.5 Airy, mode XYZ, format 512 × 512.

Observation of Solanine-induced change in the membrane potential of the mitochondria in the cells using LCSM: After 48 h, the cells were taken out of the incubator. The culture solution in the Petri dishes was sucked out, the cells were rinsed 2-3 times with PBS, and 200 μL of tetramethyl rhodamine ethyl ester (TMRE, Molecular Probes) was gently added to the dishes, so that the final concentration was 2 μmol/L (1.03 μg/mL). The cells were then incubated for 30 min and, after the staining solution was sucked out, gently rinsed 3 times with PBS. 200 μL PBS was used to cover all the cells in the troughs, and then LCSM was used to observe the fluorescence intensity of the cells, with an excitation wavelength of 543 nm and emission wavelength of 570 ± 20 nm. Other devices included objective APO CS40 ×/1.25 oil, zoom > 1, pinhole 1.5 Airy, mode XYZ, format 512 × 512.

Simultaneous observation of changes in the morphology of cells, membrane potential, and the concentration of calcium in the cells: Experiment was performed on 2 groups of HepG₂ cells simultaneously. Cell incubation, treatment, and operation procedures were all the same as above, with the same amount of time and conditions for cell incubation. One group was marked with AO/EB and the effect of solanine on its HepG₂ cells was observed using LCSM; while HepG₂ cells in the other group were marked with TMRE and Fluo-3/AM, and changes in membrane potential of the mitochondria and [Ca²⁺]_i in the cells were observed using LCSM. Methods for AO/EB double staining and measurement were as before, while the procedures for double staining with Fluo-3/AM and TMRE and measurement are as follows: (1) Staining after 48 h, the cells were taken out of the incubator. The culture solution in the Petri dishes was sucked out, the cells were rinsed 3 times with RPMI1640, and 150 μL of tetramethyl rhodamine ethyl ester (TMRE, Molecular Probes) working fluid with a concentration of 2 μmol/L (1.03 μg/mL) was pipetted onto the dishes. The cells were then incubated for 30 min at 37°C, and after the staining solution was sucked out, rinsed 3 times with RPMI1640. Finally 150 μL of Fluo-3/AM (Molecular Probes) with a concentration of 4 μg/mL was added. The cells were then incubated for 50 min at 37°C and rinsed 3 times with RPMI1640, and 200 μL of RPMI1640 was added to cover all the cells in the troughs, whereupon the Petri dishes were mounted for measurement. (2) Observation LCSM was used to examine the fluorescence intensity (FI) of the cells. Dual-wavelength excitation was used, with excitation wavelengths of 488 nm and 543 nm, respectively. The emission wavelength used for PMT1 was 555 ± 15 nm, while that for PMT2 was 570 ± 20 nm. Other devices included Objective APO CS40 × /1.25 oil, zoom > 1, pinhole 1.5 Airy, mode XYZ, format 512 × 512. Sequential scanning was used to eliminate interference due to spectral overlap.

Statistical analysis

t test was performed on data obtained from the experi-ments, with the results expressed in the format mean ± SD. P < 0.05 was taken as significant.

RESULTS

Effect of Solanine on the morphology of HepG₂ cells

After the DNA-specific fluorochromes AO and EB were used to stain HepG₂ cells, LCSM was used to observe the morphological changes of HepG₂ cells treated with solanine. From Figure 2A, it can be seen that the control was morphologically normal. The nuclei of different cells were of similar sizes, regularly shaped, and evenly stained. The more deeply colored parts of the nuclei represented heterochromatin which did not take part in transcription under normal circumstances. With solanine treatment, however, the cells showed marked morphological changes. In the groups treated with 0.0032 μg/mL and 0.016 μg/mL of solanine (Figures 2B and 2C, respectively), the cells were wrinkled, and the chromatin was concentrated and marginalized. In the group treated with 0.08 μg/mL of solanine (Figure 2D), cells with fragments and apoptotic bodies appeared, a typical sign for apoptosis. In the groups treated with 0.4 μg/mL and 2 μg/mL of solanine (Figures 2E and 2F, respectively), the number of cells containing apoptotic bodies was significantly increased. At the same time, it could be seen that high dosage of solanine was lethal to tumor cells, because with increasing dosage, the number of viable cells decreased significantly. Apoptotic bodies also appeared clearly in the positive control treated with camptothecin with the final concentration of 0.08 μg/mL.

Figure 2 Effect of solanine on the morphology of HepG₂. A: Control group, PMT1 nuclear chromatin was stained green and showed normal structure, while PMT2 showed no or only weak red fluorescence; B: Group treated with Solanine with final concentration of 0.0032 μg/mL: The cells show slightly pyknotic and crumb-shaped structures. Permeability of cell membrane has increased, so that AO and EB can enter the cells, resulting in the cells showing both green and red fluorescence; C: Group treated with Solanine with final concentration of 0.016 μg/mL: The cells show slightly pyknotic and crumb-shaped structure. Fragments or apoptotic bodies appear in the nuclei of some of the cells; D: Group treated with solanine with final concentration of 0.08 μg/mL: Cell structure is further damaged. The cells are not only stained with AO and EB, but morphologically the nuclei have become deeply stained fragments or apoptotic bodies; E: Group treated with solanine with final concentration of 0.4 μg/mL: Cell structure is damaged even further. Apoptotic bodies have definitely appeared; F: Group treated with solanine with final concentration of 2 μg/mL: Definite apoptotic bodies can be seen, while the number of cells in sight has decreased; G: Group treated with camptothecin with final concentration of 2 μg/mL: The appearance of apoptotic bodies is obvious in HepG₂ cells.

Change induced by Solanine in [Ca²⁺]_i in HepG₂ cells in the process of apoptosis

As shown in Figure 3 the effect of different dosages of solanine (0.0032, 0.016, 0.08, 0.4, and 2 μg/mL) on [Ca²⁺]_i in HepG₂ cells was observed using LCSM with staining with the fluorescent probe Fluo-3/AM. On the left side of each group is the photograph (green) showing [Ca²⁺]_i taken under the confocal microscope, with the depth of the color representing the fluorescence intensity (FI). On the right is the 3D configuration reconstructed using LCSM for the group, with different colors reflecting different FI values, which indirectly reflect the concentrations of [Ca²⁺]_i for the test group in question. It is shown from these photographs that the [Ca²⁺]_i for the control in Figure 3A was very low, while those for the groups treated with solanine (Figures 3B-F) were all increased, in a concentration dependent manner. The positive control camptothecin did not increase [Ca²⁺]_i. Statistics on FI obtained through LCSM for the various groups in Table 1 showed the same results.

Table 1 Effect of Solanine on [Ca²⁺]_i in HepG₂ cells (mean ± SD).

Group	Final concentration(μg/mL)	Number ofcells (n)	FL
Control	-	18	23.98 ± 10.06
Camptothecin group	0.08	16	26.92 ± 8.20
Solanine groups	0.003	25	40.31 ± 10.15b
	0.016	37	59.31 ± 17.56b
	0.08	17	91.01 ± 23.19b
	0.4	19	158.75 ± 14.52b
	2	20	209.85 ± 10.86b

Comparison with blank:

^bP < 0.01.

Figure 3 Effect of Solanine on [Ca²⁺]_i in HepG₂ cells. A: Control; B: 0.0032 μg/mL solanine; C: 0.016 μg/mL solanine; D: 0.08 μg/mL solanine; E: 0.4 μg/mL solanine; F: 2 μg/mL solanine; G: 0.08 μg/mL camptothecin.

Change induced by Solanine in the membrane potential of mitochondria in HepG₂ cells in the process of apoptosis

The effect of different dosages of solanine (0.0032, 0.016, 0.08, 0.4, and 2 μg/mL) on the membrane potential of mitochondria in HepG₂ cells was observed using LCSM with staining by the fluorescent probe TMRE (Figure 4). The brightness of the coloration reflected different values of FI, thus indirectly indicating the membrane potential of mitochondria in the cells for these different groups. From those photographs, it could be seen that the membrane potential of mitochondria in the cells was relatively high for the control in Figure 4A, while those for the groups treated with solanine (Figures 4B-F) were all increased, in a dosage dependent manner. Statistics on FI obtained through LCSM for the various groups in Table 2 showed that the membrane potential of mitochondria in the cells was significantly lowered (P < 0.01) in groups treated with 0.016, 0.08, 0.4, and 2 μg/mL of solanine. The membrane potential in the 0.0032 μg/mL group was also lowered, but the difference from the control was not significant. The membrane potential of mitochondria in HepG₂ cells of the group treated with 0.08 μg/mL of camptothecin was also significantly lowered.

Table 2 Effect of solanine on the membrane potential of mitochondria in HepG₂ cells (mean ± SD).

Group	Final concentration(μg/mL)	Cell number(n)	FI
Control	-	53	75.70 ± 31.24
Camptothecin group	0.08	16	47.17 ± 15.98
Solanine groups	0.003	66	70.04 ± 17.83
	0.016	90	63.21 ± 16.36b
	0.08	144	52.26 ± 26.46b
	0.4	37	32.50 ± 19.99b
	2	33	28.49 ± 9.71b

Comparison with blank:

^bP < 0.01.

Figure 4 Effect of Solanine on membrane potential of mitochondria in HepG₂ cells. A: Control; B: 0.0032 μg/mL solanine; C: 0.016 μg/mL solanine; D: 0.08 μg/mL solanine; E: 0.4 μg/mL solanine; F: 2 μg/mL solanine; G: 0.08 μg/mL camptothecin.

Simultaneous observation of changes induced by solanine in cell morphology, [Ca²⁺]_i in cells, and mitochondrial membrane potential in HepG₂ cells in the process of apoptosis

Double staining with AO/BE and with Fluo-3/AM and TMRE were simultaneously used in this experiment (Figure 5). Changes in cell morphology, [Ca²⁺]_i in cells, and membrane potential of mitochondria in the same cell were simultaneously observed using LCSM for groups treated with different concentrations of solanine, so as to examine simultaneous changes in cell morphology, [Ca²⁺]_i in cells, and membrane potential of mitochondria in cells at the same time points in the apoptosis process induced by solanine of different concentrations and, moreover, to explore the physiological mechanisms of [Ca²⁺]_i and membrane potential of mitochondria in the cells and the relationship between them. As shown in Figure 5A for the control group, the cells were normal morphologically, and the [Ca²⁺]_i in the cells was relatively low, while the corresponding mitochondrial membrane potential was relatively high. Results for the groups treated with solanine (with final concentration of 0.0032, 0.016, 0.08, 0.4, and 2 μg/mL, respectively) are shown in Figures 5B-F. It could be seen that with the dosage increased, the shape of the cells gradually became more and more irregular, and typical apoptotic cells such as irregularly shaped cells and apoptotic bodies appeared, with apoptotic bodies, characteristic of apoptosis appearing first in the group treated with 0.08 μg/mL of solanine (Figure 5D). As the dosage increased, the percentage of apoptotic bodies gradually increased, and [Ca²⁺]_i in the cells also gradually increased, while the membrane potential of mitochondria in the cells gradually decreased for the corresponding groups. The group treated with camptothecin showed no change in [Ca²⁺]_i but it did show a significantly decreased membrane potential of mitochondria, results consistent with those obtained with single staining. It also showed that solanine induced apoptosis in HepG₂ cells by a different mechanism from camptothecin. Detailed changes of different groups are shown in Table 3.

Table 3 Effect of solanine on [Ca²⁺]_i in HepG₂ cells and mitochondrial membrane potential (mean ± SD).

Group	Final concen-tration (μg/mL)	Numberof cells	Fluo-3/AM-FI	TMRE-FI
Control	-	111	24.96 ± 4.03	51.85 ± 19.18
Camptothecin group	0.08	21	28.80 ± 7.01	25.50 ± 5.76b
Solanine groups	0.003	49	33.85 ± 9.30a	39.52 ± 15.41b
	0.016	33	34.37 ± 14.18a	35.01 ± 11.12c
	0.08	36	42.73 ± 14.75b	28.13 ± 4.31d
	0.4	39	52.80 ± 11.07b	23.21 ± 12.22d
	2	37	58.62 ± 16.04b	13.09 ± 4.71d

Comparison with blank:

^aP < 0.05;

^bP < 0.01;

^cP < 0.005;

^dP < 0.001.

Figure 5 Changes induced by solanine in cell morphology, [Ca²⁺]_i in cells, and membrane potential of mitochondria in HepG₂ cells in the process of apoptosis. A: Control; B: 0.0032 μg/mL solanine; C: 0.016 μg/mL solanine; D: 0.08 μg/mL solanine; E: 0.4 μg/mL solanine; F: 2 μg/mL solanine; G: 0.08 μg/mL camptothecin.

Solanine-induced changes in [Ca²⁺]_i and membrane potential of mitochondria in HepG₂ cells in the process of apoptosis and their distribution in the cells

When the images for the double staining with Fluo-3/AM and TMRE were recombined using the image analyzing capacity of the laser confocal scanning microscope, it could be seen that Ca²⁺ was widely distributed in the whole cell, but with different concentrations in different regions of the cell. In contrast, membrane potential of mitochondria was distributed only in certain parts of the cell (Figure 6). Superimposing the two images, it can be seen that regions where Ca²⁺ was more concentrated were basically also those where membrane potential was distributed.

Figure 6 Distribution of solanine-induced changes in [Ca²⁺]_i and membrane potential of mitochondria in the HepG₂ cells in the process of apoptosis. A: Blank; B: 0.0032 μg/mL solanine; C: 0.016 μg/mL solanine; D: 0.08 μg/mL solanine; E: 0.4 μg/mL solanine; F: 2 μg/mL solanine.

DISCUSSION

The mitochondrion is an important organelle of the eukaryotic cell and the main site for the production of ATPs in animal cells[18,19]. NADH is produced from the tricarboxylic cyclophorase series in mitochondrial matrix through the dehydroxidization of the substrate, and then oxidized through the electron transport chain on the inner membrane of the mitochondrion. This leads to the transmembrane transfer of protons, resulting in a transmembrane proton gradient (or transmembrane potential). The ATP synthetase on the inner membrane of the mitochondrion synthesizes ATPs using the energy derived from the proton gradient. Through the ADP/ATP carriers on the inner membrane of mitochondria, ATPs synthesized this way are exchanged for ADPs from the cytoplasm, thus entering the cytoplasm to take part in various processes requiring energy.

In recent years, reports have been made that the dissipation of the membrane potential of mitochondria precedes the activation of nuclease[20], and that it precedes the exposure of phosphatidylserine on the surface of the cell. When the transmembrane potential of mitochondria is dissipated, the cell would enter the irreversible apoptotic process. The uncoupled respiratory chain of the mitochondrion would produce a large amount of active oxygen, oxidizing the cardiolipin on the inner membrane of the mitochondrion. Experiments have shown that the decoupling reagent mClCCP can lead to the apoptosis of lymphocytes[21,22], whereas if the transmembrane potential of mitochondria can be stabilized, apoptosis would be prevented. Therefore, the dissipation of transmembrane potential of mitochondria is closely related to apoptosis.

The dissipation of transmembrane potential of mitochondria in the process of apoptosis is mainly caused by changes in the permeability of the inner membrane of mitochondria, which is due to the formation of dynamic pores for permeability transition (PT) (each composed of several proteins) at points of contact between the outer and inner membranes of the mitochondrion[23]. PT channels are made up of proteins from various parts of the mitochondrion and those from the cytoplasm[24,25]. They include a protein in the cytochylema (hexokinase)[25], proteins on the outer membrane of the mitochondrion (peripheral benzodiazepine receptors and voltage-dependent anion channels), a protein in the interstice between the inner and outer membranes of the mitochondrion (creatine kinase)[26], a protein on the inner membrane of the mitochondrion (the ADP-ATP carrier)[27], and a protein in the mitochondrial matrix (cyclophilin D)[28]. Any substance, such as protoporphyria IX, the ligand for benzodiazepine receptors, that can specifically induce the formation of PT channels in mitochondria could cause apoptosis[29-32]. PT channels are a kind of channels with high electric conductivity. The lowering or even disappearance of membrane potential would suggest that PT channels are open.

Results from earlier experiments show that solanine can induce the apoptosis of HepG₂ cells. In the present study, double staining with AO/EB and solanine (0.0032, 0.016, 0.08, 0.4, and 2 μg/mL) carried by TMRE were used to treat HepG₂ cells, and LCSM was used to observe changes in the membrane potential of mitochondria as solanine induced the appearance of apoptotic bodies[33]. As solanine induced the appearance of apoptotic bodies and caused the apoptosis of cells, the membrane potentials of mitochondria in cells in groups treated with different dosages of solanine (0.0032, 0.016, 0.08, 0.4, and 2 μg/mL) were all decreased in comparison with the control (Figures 2 and 4, and Table 2). The amount of decrease was dosage-dependent, and the difference from that of the control was significant for the groups treated with 0.016, 0.08, 0.4, and 2 μg/mL of solanine, respectively, suggesting that solanine can lead to the lowering of the membrane potential of mitochondria in HepG₂ cells. However, the lowering of the membrane potential of mitochondria is a manifestation of the opening of the PT pores of the mitochondria[34], so solanine can lead to the opening of the PT pores of the mitochondria.

The opening of PT pores can lead to the occurrence of two events[35]: (1) the intra-membrane and extra-membrane ion concentrations tend toward equilibrium, the transmembrane H⁺ gradient disappears, and the respiratory chain is uncoupled; (2) the flow of intra- and extra-membrane ions toward equilibrium leads to a hypertonic mitochondrial matrix, resulting in change in the volume of the mitochondrion. Since the surface area of the folded inner membrane of mitochondrion is larger than that of the outer membrane, this would lead to the rupture of the outer membrane.

These two events lead respectively to two conse-quences. Since a large number of Ca²⁺ have accumulated in mitochondria, the concentration of Ca²⁺ in mitochondria is much higher than that in the cytoplasm. The occurrence of the first event would lead directly to the flow of Ca²⁺ from the mitochondria to the cytoplasm, resulting in rapid increase in the concentration of Ca²⁺ in the cytoplasm. In the present study, the Ca²⁺-specific molecular probe Fluo-3/AM was used to carry solanine at different concentrations to treat HepG₂ cells, and LCSM was used to observe changes in [Ca²⁺]_i in the cells after the treatment. In groups treated with different dosages of solanine (0.0032, 0.016, 0.08, 0.4, and 2 μg/mL), the concentrations of Ca²⁺ in HepG₂ cells were all increased in a dosage-dependent way (Figure 3 and Table 1). This verifies the inference we have drawn, namely, by opening up PT channels, solanine leads to the release of Ca²⁺ from the mitochondria, resulting in the increase of [Ca²⁺]_i in the cell. In order to obtain more powerful evidence for our inference, LCSM with double staining with Fluo-3/AM and TMRE was used to observe simultaneously the change in membrane potential and the change in [Ca²⁺]_i in the cell after the treatment. The results showed that as the dosage increased, the rate of apoptosis gradually increased, the membrane potential gradually decreased, and [Ca²⁺]_i in the cell gradually increased (Figure 5 and Table 3). These results were consistent with that obtained with single staining.

At the same time, the data collected using LCSM was analyzed and observation was made on the distribution of [Ca²⁺]_i and the membrane potential of mitochondria in the cells in the apoptotic process of HepG₂ induced by solanine. The image overlay function of LCSM was used to superimpose and compare the images of [Ca²⁺]_i and the membrane potential of mitochondria in the cells. Results showed that membrane potential was mainly distributed on the inner membrane of the cells, with no distribution in the center of the cells; but the inner membrane was precisely where mitochondria were concentrated (Figure 6). [Ca²⁺]_i was distributed throughout the whole cell, but the distribution was uneven. This can be observed in the photograph with green fluorescence in Figure 6, where the brightness of the fluorescence depends on the region of the cell. When the image for [Ca²⁺]_i distribution (green) was superimposed on that for membrane potential (red), it can be seen that regions where Ca²⁺ was concentrated overlaps with where membrane potential was concentrated (as shown by the yellow regions in the third photograph in each group in Figure 6), suggesting that the distribution of Ca²⁺ in the mitochondria was high. In addition, the concentration of Ca²⁺ in both the mitochondria and the cytoplasm continued to rise in the different groups, suggesting that as [Ca²⁺]_i increased in the cytoplasm, Ca²⁺ flew out not only from the mitochondria, but also from some other source(s) as well, e.g., an inflow of Ca²⁺ from outside the cell.

In conclusion, solanine can facilitate the opening of the PT channels in mitochondria, leading to release of Ca²⁺ from these organelles. This results in an increase in the concentration of Ca²⁺ in the cell, thus triggering the mechanism for apoptosis and the occurrence of apoptosis. However, to uncover how the PT channels are opened and which enzymes and genes are involved in this process, further research is needed.