A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice

Figure 3 Transgene expression investigated by Northern blot analysis. A: Map of the transplastomic genome with areas to which different probes hybridize. Lengths of possible transcripts are shown in kb. B: RNA blot analysis for characterization of different transcripts. RNA blots were hybridized with probes targeting the aadA-coding region (P4), HEV pE2-coding sequence (P5), junction between the rice psbA terminator and tobacco rrn promoter (P6) and tobacco trnfM (P7). Arrows to the left of the panels denote lengths of RNA in kb. Normalization of RNA loading was demonstrated by ethidium bromide staining of the RNA gel.