A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice

Figure 1 Transplastomy of the spectinomycin-resistant plants. A: Maps of WT and transplastomic genomes in the area of two arms (psaB-trnfM and trnG-psbC) for homologous recombination. Horizontal arrows indicate locations and orientations of different primers. The sizes of PCR products are shown in kb. Arrows pointing upwards denote nucleotide positions in the tobacco chloroplast genome (TCG) in accordance with GenBank accession no. Z00044. PRpsbA: rice psbA promoter; TRpsbA: rice psbA terminator; PTrrn: tobacco rrn promoter; TTpsbA: tobacco psbA terminator. B: Agarose gel pictures of PCR products. Primers used for generating each product are given to the right of the panels. Arrows indicate sizes of the PCR products in kb. Primer pair Hel-19/Hel-24 amplified a 1.4-kb fragment in the rbcL gene presenting in both the WT and the transplastomic plants.