An efficient method of sorting liver stem cells by using immuno-magnetic microbeads

Figure 5 Hepato- and cholangiocyte- specific gene expressions of liver cells. The mRNA was prepared and RT-PCRs were performed for the genes displayed in the figure in each cell subset. β-actin (Actin) gene expression was used as controls for mRNA content per sample. A representative experiment for all the gene expressions of a total of three independent ones is shown. ML, mature mouse liver cells; ED13, ED 13 mouse fetal liver cells; FLSC, fetal liver stem cells [c-Kit^-(CD45/TER119)^- cells]; FLSC-5d, FLSC cultured for 5 d; FLSC-21d, FLSC cultured for 21 d.