An efficient method of sorting liver stem cells by using immuno-magnetic microbeads

Figure 4 In vitro subcolony formation from the sorted c-Kit^–(CD45/TER119)^– cell. Culture of c-Kit^–(CD45/TER119)^– cells was performed on laminin-coated 24-well plates for 4 d. Colonies were then digested and the cells from the formed clone were plated onto new culture plates and cultured for another 5 d. The picture shows a representative subcolony.