Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract

Table 2 PCR-primers, expected fragment sizes and PCR amplification conditions

Primer	Sequence, 5’ to 3’ orientation	Size in bp	PCR conditions
			1Cycles	Tannealing
hGRP-SE/1	AGTCTCTGCTCTTCCCAGCCTCT
hGRP-AS/1	GCAGAACTCAGTCTCTTAGGGGT	558	30	55 °C
hGRP-SE/2	CGTGCTGACCAAGATGTACC
hGRP-AS/2	TCATTGCTGGTTCAGCTGGG	349	30	62 °C
hGRPR-SE/1	AGCCCGGCATAGATCTTATCTTC
hGRPR-AS/1	AGGGGGCAAAATCAAGGGTCAAT	1477	30	55 °C
hGRPR-SE/2	CTCCCCGTGAACGATGACTGG
hGRPR-AS/2	ATCTTCATCAGGGCATGGGAG	388	30	62 °C
β-actin-SE	GCATGGAGTCCTGTCGCATCCACG
β-actin-AS	CGTCATACTCCTGCTTGCTGATCCA	2288/400	30/25	55 °C

¹Numbers of cycles in first and second round PCR amplifications;

²cDNA and gDNA PCR amplicon sizes, respectively.