Mechanisms inactivating the gene for E-cadherin in sporadic gastric carcinomas

Figure 4 Promoter hypermethylation of the CDH1/E-cad detected by methylation-specific PCR (MSP). The presence of a visible PCR product in the lanes marked U indicates the presence of an unmethylated allele, while the presence of the product in the lanes marked M indicates the presence of a methylated allele. The intensity of each methylated band was further semi-quantitated, and as shown in the figure, cases 1 and 4 were defined as “hypermethylation”with “+”and “++”, respectively, and cases 2 and 3 were defined as “unmethylation”.