Mechanisms inactivating the gene for E-cadherin in sporadic gastric carcinomas

Figure 3 Allelic loss or loss of heterozygosity (LOH) of CDH1/E-cad. Left panel: E-cad detected by allelic loss or loss of heterozygosity (LOH) of the E-cad locus, reflected by three microsatellite markers (D16S3043, D16SS3050 and D16S3021) at 16q22.1. Right panel: LOH in a representative GC. The locius of markers D16S3043, D16S3050, and D16S3021 were considered to be informative when they were heterozygous in normal tissue (i.e. two alleles were seen), and showed LOH when a 3-fold or greater difference was seen in the relative allele intensity ratio between the tumor and normal DNA (arrow).