Helicobacter pylori and other Helicobacter species DNA in human bile samples from patients with various hepato-biliary diseases

Table 2 Conditions of polymerase chain reactions used in the study

Target gene	Initial denaturation step	Temperature o denaturation, annealing and	Cycle	Final extension Step extension
Helicobacter	94 °C for 5 min	94°C for 30 s
Spp,16S rRNA		55°C for 30 s	35	72°C for 7 min
				72°C for 1.5 min
Second	94°C for 5 min	94°C for 30 s
amplification		60°C for 30 s	35	72°C for 7 min
step		72°C for 30 s
Helicobacter	95°C f	94°C for 30 s
pylori, 16S rRNA		56°C for 30 s	40	72°C for 5 min
				2°C for 1 min
ureA	95°C for 5 min	94°C for 30 s	35	72°C for 6 min
		52°C for 30 s		72°C for 1 min
cagA	95°C for 5 min	94°C for 1 min
		52°C for 1 min	35	72°C for 10 min
		72°C for 2 min