Adeno-associated virus-mediated heme oxygenase-1 gene transfer suppresses the progression of micronodular cirrhosis in rats

Figure 4 A: The representative pictures of pro-inflammatory and pro-fibrogenic responses in the livers of rats with or without treatment at the 10^th week. Infiltrating macrophage (ED1), tissue residential macrophages (ED2), TGF-β1, and activated hepatic stellate cells (desmin) were detected by immunohistochemistry; original magnification, ×100; B: The profile of pro-inflammatory and profibrogenic cytokine expression. The level of mRNA was detected by RNase protection assay and was quantified by phosphoimager, n = 3-5, ^aP<0.05; C: The plasma level of macrophage migration inhibitory factor (MIF) was detected by ELISA. The data were shown as fold changes of over the level in normal rat, n = 3-5, ^bP < 0.001.