Adeno-associated virus-mediated heme oxygenase-1 gene transfer suppresses the progression of micronodular cirrhosis in rats

Figure 2 A: The model to induce micronodular cirrhosis in LEW rat. The rAAVs were injected through the portal vein shortly before the induction; B: The histology and immunohistochemistry of the livers with or without treatment at the 10^th week (H-E, original magnification ×40; MT: Masson’s trichrome stiaining, the fibrotic elements were stained in green, original 100×; collagen 1 and fibronectin staining, original magnification ×100); C: The measurement of fibrotic area in the livers at the 10^th week by the computer software as described in Materials & Methods. Data were shown as mean±SE, n = 5, ^bP < 0.001; D and E: The systolic and portal pressure of rats after induction of liver cirrhosis with or without treatments. The data were shown as mean±SE, n = 10, ^dP < 0.001.