Basic Research
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 28, 2006; 12(12): 1889-1894
Published online Mar 28, 2006. doi: 10.3748/wjg.v12.i12.1889
Figure 3
Figure 3 Expression and quantitative analysis of human IL-2. A: RT-PCR of in vitro cultured BMSC. Lane 1: 1x106 stromal cells; lane 2: 1x106 stromal cells containing 20 MOI Ad/∆E1; lane 3: 1x106 stromal cells containing 20 MOI Ad/hIL-2; B: ELISA assay of IL-2 secretion from in vitro cultured BMSCs (2.5 X 105/mL) of Syrian golden hamster after transduction with Ad/hIL-2 at MOI of 0(◆), 25(■), 50(▲), and 100 (●). Culture supernatants were harvested on different days and tested for hIL-2 secretion levels by ELISA.