Reconstruction of liver organoid using a bioreactor

Figure 3 Transmission electron microscopic images of cocultures in the RFB. A: The cells are arrayed on the cellulose beads. Several cell clusters could be seen in a gap of cellulose beads (arrow). Vascular lumen structure surrounding cell clusters could be seen in the beads (small arrow). Culture media flow through inside of lumen structure; B: The cells are arrayed in layers on cellulose beads. Part of a cellulose bead (arrow) is visible at the bottom of the layer. A process of a sinusoidal endothelial cell (arrowhead) is seen at the perfusion side. Scale bar: 5 μm; C: Sinusoidal endothelial cells (EC) can be seen at the perfusion side. Hepatic stellate cells (SC) containing fatty vitamin A droplets are seen overlying the FLC-5 cells (H). FLC-5 cells (H) below EC and SC show bile-canaliculus-like structures (B). Scale bar: 5 μm. D: Bile canaliculus-like structures (B) containing electron dense bile components. Tight junctions (t) and desmosomes (d) are visible, as are fatty vitamin A droplets (L). Scale bar: 2 μm.