Glycine-extended gastrin activates two independent tyrosine-kinases in upstream of p85/p110 phosphatidylinositol 3-kinase in human colonic tumour cells

Figure 6 No involvement of p60-Src (A) and JAK2 (B) activation in response to G-gly. Cells pretreated with or without PP2 and AG 490 (10 µmol/L), were stimulated (+) or not (-) by G-gly (10 pmol/L) for 5 min. JAK2 and p60-Src kinase activities were determined by immune complex kinase assay using anti-JAK2 and anti-p60-Src antibodies as described in Materials and Methods. Proteins were separated by SDS-PAGE and JAK2 autophosphorylation was detected by autoradiography. A representative autoradiogram from at least three separate experiments is shown.