Glycine-extended gastrin activates two independent tyrosine-kinases in upstream of p85/p110 phosphatidylinositol 3-kinase in human colonic tumour cells

Figure 5 Involvement of PI3K (A) and JAK2 (B) activation in response to G-gly. HCT116 cells pretreated with or without 10µmol/L of LY290042 and were 10µmol/L of AG 40, stimulated (+) or not (-) with glycine-extended gastrin G-gly (10 pmol/L) for the indicated time. After immunoprecipitation of cell lysates with anti-JAK2 and anti-p85 antibody, precipitates were assayed for PIK activity using inositol phosphate as an exogenous substrate. Lipids were separated by thin layer chromatography and autoradiographied. The result shown is representative from five separate experiments.