Glycine-extended gastrin activates two independent tyrosine-kinases in upstream of p85/p110 phosphatidylinositol 3-kinase in human colonic tumour cells

Figure 4 Involvement of p60-Src (A) and PI3K (B) activation in response to G-gly. HCT116 cells pretreated with or without 10 µmol/L of LY290042 and anti-p85 antibody were stimulated (+) or not (-) with glycine-extended gastrin (10 pmol/L) for the indicated time. After immunoprecipitation of cell lysates with anti-p60-Src and anti-p85 antibody, precipitates were assayed for PIK activity using inositol phosphate as an exogenous substrate. Lipids were separated by thin layer chromatography and autoradiographied. The result shown is representative from five and three separate experiments.