Glycine-extended gastrin activates two independent tyrosine-kinases in upstream of p85/p110 phosphatidylinositol 3-kinase in human colonic tumour cells

Figure 1 G-gly-induced PI3K (A) and G-gly (B) activation. HCT116 cells were stimulated with G-gly (10 pmol/L) for the indicated time. PI3K activity was determined in an immune complex kinase assay as described in Methods. The specificity of G-gly activation was accessed by pretreating the cells with ferric chelator DFO (1µmol/L). Representative autoradiograms from at least three separate experiments are shown.