Melanoma differentiation-associated gene-7, MDA-7/IL-24, selectively induces growth suppression, apoptosis in human hepatocellular carcinoma cell line HepG2 by replication-incompetent adenovirus vector

Figure 2 Ad. mda-7 expression causing in vitro inhibition of growth of HCC cells, but not normal liver cells. The various cell types were uninfected (control) or infected with 1 000 VP/cell of Ad.vec or Ad.mda-7. Cell numbers were determined over an 8-d period. Cells were seeded in 96-well plates and treated the next day as described in ‘Materials and Methods’. After 24 h, the medium was removed, and cells were stained with MTT. Experiments were repeated three times in quadruplicates, and the results were presented as mean±SE.