Melanoma differentiation-associated gene-7, MDA-7/IL-24, selectively induces growth suppression, apoptosis in human hepatocellular carcinoma cell line HepG2 by replication-incompetent adenovirus vector

Figure 1 Expression of mda-7/IL-24 mRNA. Infection of normal human liver cells and HCC with Ad.mda-7 resulted in an expression of mda-7/IL-24 mRNA. Cells infected with 1 000 VP/cell of Ad.vec or Ad.mda-7 were harvested at 48 h, treated as described in “Materials and Methods”. The total RNA was extracted and the RT-PCR was performed. (A) Lane 1: marker; lane 2: control L02 cells; lane 3: Ad.vec-infected L02 cells; lane 4: Ad.mda-7-infected L02 cells; lane 5: control HepG2 cells; lane 6: Ad.vec-infected HepG2 cells; lane 7: Ad.mda-7-infected HepG2 cells. (B) Expression of β-actin.