Cis-hydroxyproline-induced inhibition of pancreatic cancer cell growth is mediated by endoplasmic reticulum stress

Figure 6 Reversion of CHP-mediated molecular effects by L-proline. A: RT-PCR analysis of mRNA expressions of GRP78 and GADD153. Lane 1: DSL6A cells cultured without 10 mmol/L CHP for 6 h; lane 2: DSL6A cells cultured with 10 mmol/L CHP for 6 h; lane 3: DSL6A cells cultured with 10 mmol/L CHP for 24 h; lane 4: DSL6A cells treated for 24 h with CHP after the addition of 20 mol/L L-proline for further 6 h; lane 5: DSL6A cells treated for 24 h with CHP after the addition of 20 mol/L L-proline for further 24 h. B: Western blot analysis of GRP78 and FAK protein. Results are representative of at least three independent experiments.