Reduced expression of β-catenin inhibitor Chibby in colon carcinoma cell lines

Figure 3 Expression of Chibby in transfected SW480 cells. A: Western blot analysis showing Chibby protein expression levels in untransfected (mock), control vector, or Chibby transfected SW480 cells. B: immunofluorescence staining of flag-tagged Chibby transfected SW480 cells. Nuclei were stained with DAPI in blue, flag-tagged Chibby was detected with FITC-labelled anti-flag antibody (green). C: TOPflash reporter gene assays showing inhibition of β-catenin signalling after transfection of SW480 cells with Chibby expression plasmid.