Reduced expression of β-catenin inhibitor Chibby in colon carcinoma cell lines

Figure 1 PCR amplification of the complete coding region of Chibby mRNA. The Chibby coding region was amplified using RT-PCR. All colon carcinoma cell lines were shown to express full-length Chibby mRNA. Expression levels cannot be estimated from this experiment as the PCR was carried out to saturation in order to illustrate full-length mRNA expression and to obtain enough PCR products for sequencing. β-actin amplification served as control to ascertain the integrity of the cDNA in all samples.