Berberine induces cell cycle arrest and apoptosis in human gastric carcinoma SNU-5 cell line

Figure 1 Percentage of viable SNU-5 cells treated with berberine with 24-h incubation. The SNU-5 cells (2×10⁵ cells/well; 12-well plate) were plated in 80% Iscove’s modified Dulbecco’s medium+20% FBS with different concentrations of berberine for 24 h (Panel A) or 100 µmol/L berberine for 6, 12, 24, 48, and 72 h (Panel B). Then the cells were collected by centrifugation and the viable cells were determined by trypan blue exclusion and flow cytometry as described in Materials and Methods. Each point is mean ± SD of three experiments.