Differential phosphorylation of p38 induced by apoptotic and anti-apoptotic stimuli in murine hepatocytes

Figure 2 TPA and EGF block TGF-β1-induced apoptosis. AML-12 cells were incubated with TGF-β1 (5 ng/mL) in the presence or absence of TPA (50 ng/mL) as indicated for 18 h or in the presence or absence of EGF (50 ng/mL) as indicated for 24 h. A and C: The cells were stained with AO/EB (400×magnifications); B and D: Apoptotic rate was determined by flow cytometry analysis.