Differential phosphorylation of p38 induced by apoptotic and anti-apoptotic stimuli in murine hepatocytes

Figure 1 The role of p38 in TGF-β1-induced apoptosis in AML-12 hepatocytes. A: AML-12 cells were treated with TGF-β1 (5 ng/mL) for the indicated time, and the phosphorylation of p38 was examined with anti phospho-p38 antibody; B, C and D: Cells were treated with TGF-β1 (5 ng/mL) for 24 h in the presence or absence of SB202190 (10 μmol/L). Apoptotic rate was determined by measuring DNA content with flow cytometry analysis (B); the caspase-3 activity (C); and DNA fragmentation assay (D).