Identification of differentially expressed genes after partial rat liver ischemia/reperfusion by suppression subtractive hybridization

Figure 8 Hsp70 protein localization in rat livers after WI/R (paraffin-embedded sections). Hsp70 staining of paraffin-embedded liver tissues after partial WI/R. Up to 2 h of reperfusion Hsp70 was located in sinusoidal cells and cholangiocytes, connective cells, endothelial cells of portal areas only in the I-lobes (I-lobes after 1 h/2 h WI/R: B+D), while Hsp70 was not detectable in the C-lobes at these time points (C-lobes after 1 h/2 h WI/R: A+C). Parenchymal Hsp70 staining was only noticed after 6 h reperfusion (C-lobe: E; I-lobe: F+H).Comparison of perivenular vacuolization (HE staining: G) and Hsp70 protein expression in the affected hepatocytes after 1 h/6 h WI/R (H).