Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells

Figure 7 Dose dependent inhibition of HBsAg expression in HepG2 cells. 0, 5, 15 or 50 ng siHBVP was co-transfected with 0.35 μg pUC-HBV2 and 0.35 μg pSEAP2-control into HepG2 cells. The supernatant of culture media were removed every 24 h and fresh media were added. The HBsAg and alkaline phosphatase secreted into the culture media were measured. A and C show the HBsAg or SEAP expression level represented by OD values. B shows the HBsAg expression level relative to the positive control. Values represent averages of three independent experiments, with the error bars indicating the standard deviation.