Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells

Figure 5 Specific inhibition of GFP expression in SMMC7721 cells. 0.4 μg pEGFP-N2 plasmid DNA were co-transfected with 0.4 μg pSEAP2-control plasmid DNA (A, D) or 0.4 μg siGFP (B, E), or 0.4 μg siAP (C, F) into the SMMC7721 cells in a 24-well plate. Cells were photographed 48 h post transfection. 0.4 μg pSEAP2-control plasmid was added in control group to ensure that transfection parameters remain the same. The total amount of nucleic acids was 0.8 μg for each group and the package efficiency of siRNAs and plasmid DNAs with lipofectamine 2000 was assumed to be equal. The fluorescent and bright-field pictures (D–F) show that the cell density was the same for all groups. Three independent experiments were performed, and one typical picture was shown.