Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells

Figure 4 Processing of long dsRNAs into siRNAs with recombinant E. coli RNase III. A: 1, 3 and 5 μg dsRNAs (Lanes 2-4) were digested with 1 µg RNase III at 37 °C for 1 h in 10 µg reaction mixture containing 50 mmol/l Tris–HCl, 50 mmol/L NaCl, 10 mmol/L MnCl₂ and 1 mmol/L DTT (pH 7.5). The siRNA-like products were indicated by an arrow on the right. Lane 1: undigested dsRNAs; B: The siRNAs were recovered from the gel (Lane 2).