Identification of differentially expressed genes after partial rat liver ischemia/reperfusion by suppression subtractive hybridization

Table 1 PCR primers and annealing temperatures used for target amplification and labelling ([α-³²P]-dCTP).

Description	Accession number	Primer sequence (5'-3')	Size1 (bp)	Annealingtemperature (°C)
Adapter-1		TCGAGCGGCCGCCCGGGCAGGT		68
Adapter-2R		AGCGTGGTCGCGGCCGACGT
β-Actin forward	V01217	2161-ATGTTTGAGACCTTCAACAC-2180	483	56
β-Actin reverse	V01217	2743-AACGTCACACTTCATGATGG-2724
Fos forward	XM_234422	695-TGGTGAAGACCATGTCAGGCG-715	404	60
Fos reverse	XM_234422	1098-ATTGAGAAGAGGCAGGGTGAAGG-1076
Jun forward	NM_021835	367-GGAAACGACCTTCTACGACGATG-389	519	60
Jun reverse	NM_021835	885-GGGTTGAAGTTGCTGAGGTTGG-864
Jund forward	NM_138875	465-AGTACGCAGTTCCTCTACCCTAA-487	413	58
Jund reverse	NM_138875	877-ATGTCGATGGGCGACAGTGGA-857
Hsp27 forward	M86389	157-GTTTCCCGATGAGTGGTCTCAGT-179	378	63
Hsp27 reverse	M86389	534-TCAGGGGACAGGGAAGAGGACAC-512
Hsp60 forward	X54793	913-CCAGGGTTTGGGGACAACAGG-933	380	59
Hsp60 reverse	X54793	1293-TCTTGTAGCATTGAGAGCATCTG-1271
Hsp70 forward	L16764	2129-GGCTAGAGACAGACTCTTGATGG-2151	276	58
Hsp70 reverse	L16764	2404-CTCAGTTTGTAGGGATGCAAGG-2383
Gadd45a forward	L32591	51-GAAGATCGAAAGGATGGACACGG-73	307	63
Gadd45a reverse	L32591	357-GCTCTCAGCGGGGCTCTTGTC-337
Ldlr forward	X13722	1337-CTGTGGGTTCCATAGGGTTTCTG-1359	561	60
Ldlr reverse	X13722	1897-GTTTGGAATCAACCCAATAGAGG-1875