Antidiabetic thiazolidinediones induce ductal differentiation but not apoptosis in pancreatic cancer cells

Figure 5 TZD-induced ductal differentiation in pancreatic adenocarcinoma cells. A: Effect of RGZ on the expression of differentiation markers in Panc-1 cells. After 24 h of plating, cells were incubated with 20 μmol/L of RGZ for the indicated time intervals. Protein extracts were then extracted and separated by Western blotting as described in Methods. Immunoreactive proteins were detected by incubating the filters with the specific primary antibodies. A representative of three independent experiments, yielding similar results, is shown; B: Effect of RGZ on tumor cells morphology. Panc-1 cells were plated onto sterile culture chambers and then treated with 20 μmol/L of RGZ for 96 h. After the incubation period cells were photographed with Axiovert 200 Image System (Zeiss, Gottingen, Germany). Original magnification ×200.