Spatial organization of bacterial flora in normal and inflamed intestine: A fluorescence in situ hybridization study in mice

Table 2 Spatial organization of intestinal bacteria based on FISH with Eub338 universal bacterial probe.

	Group	Ileum	Cecum	Proximal	Middle	Distal	Rectum
Mean lumen width in	WT	Narrow 0.2	Broad 6.4	Narrowing 2.9	Narrow <0.1	Widening 2.4	Broad 2.5
microscopic fields	DSS	Narrow 0.1	Broad 3.0	Irregular 0.9	Irregular 0.8	Irregular 0.6	Irregular 0.6
(×1000) between	IL-10 C-	Narrow 0.1	Broad 2.1	Irregular 0.6	Irregular 0.5	Irregular 0.7	Irregular 1.2
opposite walls	IL-10 C+	Narrow 0.3	Broad 2.0	Irregular 0.6	Irregular 0.5	Irregular 0.5	Narrow 0.2
Min -max	WT	0.2-2.5×107	>1012	>1012	-	>1012	>1012
concentrations of	DSS	<107	1.3-6.7×109	1.2-4.0×109	2.1-6.6×109	1.8-2.2×109	1.1-2.2×109
bacteria per mL	IL-10 C-	<107	1.0-5.3×1010	1.0-5.2×1010	0.6-3.4×1010	1.2-1.9×1010	1.4-2.3×1010
of feces	IL-10 C+	<107	1.7-5.0×109	0.7-4.2×109	0.7-4.2×109	4.7-7.1×109	4.7-9.2×109
Min -max. length	WT	None	None	8-10%	80-90%	Complete	Complete
of mucus gap	DSS	None	8-20%	40-50%	Complete	Complete	Exudate
separating feces	IL-10 C-	None	5-10%	70-80%	Complete	Complete	Complete
from epithelium	IL-10 C+	None	10-20%	50-80%	Complete	Complete	Exudate
Min-max width	WT	-	0	0- 5	0-20	20-50	30-100
of the mucus in	DSS	-	0- 5	0-10	10-50	40-75	60-150
µm between	IL-10 C-	-	0-10	0-10	15-50	40-100	60-200
epithelium and feces	IL-10 C+	-	0-20	0-20	5-50	60-200	Infiltrate
Mean percent of	WT	-	10% (3-12)	16% (6-16)	5% (single)	<1% (single)	0
crypts with bacteria	DSS	-	22% (4-25)	44% (9-30)	27% (4-15)	16% (2-9)	<1%
(mean-max number of	IL-10 C-	-	<1% (single)	5% (1-3)	2% (single)	1% (single)	No
bacteria within each)	IL-10 C+		28% ( 3-20)	34% (8-34)	27% (2-20)	16% (3-12)	<1%
Min-max thickness	WT	-	0	5-25	0-10	0	0
of the interlaced	DSS	-	0-100	80-400	8-40	0-2	0-2
layer (µm)	IL-10 C-	-	2-10	<5	0	0-2	0
	IL-10 C+		0-120	200-500	80-150	0-15	Infiltrate

Mean number of bacteria within crypts were counted in two different microscopic fields. Only crypts with bacteria were evaluated when mean number of bacteria within crypts was determined.