Molecular mechanisms of apoptosis induced by Scorpio water extract in human hepatoma HepG2 cells

Figure 4 Inhibition of SWE-induced caspase-3 activation (A), PARP cleavage (B) and DNA fragmentation (C) by caspase-3 inhibitor or MPT inhibitor. HepG2 cells were pretreated with Ac-DEVD-CHO (25 μmol/L) for 1 h or CsA (5 μmol/L) for 30 min followed by treatment with 2.0 mg/mL SWE for further 24 h. Lane M: DNA marker, lane 1: control, lane 2: 2.0 mg/mL SWE, lane 3: 2.0 mg/mL SWE with 5 μmol/L CsA, lane 4: 2.0 mg/mL SWE with 25 μmol/L Ac-DEVD-CHO. ^bP<0.01 vs control; ^dP<0.01 vs SWE alone.