Combination of telomerase antisense oligonucleotides simultaneously targeting hTR and hTERT produces synergism of inhibition of telomerase activity and growth in human colon cancer cell line

Figure 1 Inhibition of telomerase activity by telomerase ASODN. A: The relative telomerase activity (% control) of SW480 cells transfected with 0.2 µmol/L ASOND for 24, 48, 72 h respectively. Extracts of SW480 cell were quantified with a TRAPEZE^® ELISA telomerase detection kit. Sensitivity of telomerase PCR-ELISA (mean±SD of three experiments). B: Telomerase activity (% control) of SW480 cells transfected by ASODN with various concentrations for 72 h. The concentration of ASODN was 0.05, 0.1, 0.2 µmol/L, respectively (mean±SD of three experiments). C: PAGE figure of TRAP-PCR: Lane 1, Oligofectamine; lane 2, S-hTR; lane 3, anti-hTR; lane 4, anti-hTERT; lane 5: S-hTERT; lane 6, control; lane 7, anti-hTR+anti-hTERT; lane 8, negative; ITAS was the 36-bp internal control. Negative was lysis buffer.