Basic Research
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Feb 14, 2005; 11(6): 823-830
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.823
Table 3 Steps for measuring the activity of the oxidoreductase NADH-cell pigment C.
Reagent (mL)No saponin added (-S)
Saponin added (+S)
BlankSampleBlankSample
0.6 mg protein/mL Membrane suspension0.60.60.60.6
PBS (5p8.4)2.42.22.22
0.1% saponin//0.20.2
5 mmol/L K3Fe(CN)6/0.1/0.1
Let the solution stand for 10 min, added NADH, and recorded the result after 1 min 6 mmol/L NADH/0.1/0.1