Effect of Haimiding on the functioning of red cell membrane of FC and H22 tumor-bearing mice

Table 2 Steps for measuring the activity of the sialic acid content on the surface of the red cell membrane.

Reagent	Blank	1	2	3	4	5	6	7
N-acetyl neuraminic acid Standard solution (mL)	0	0.05	0.1	0.2	0.3	0.4	0.5	0.8
N-acetyl neuraminic acid (mg)	0	5	10	20	30	40	50	80
Distilled water (mL)	1	0.95	0.9	0.8	0.7	0.6	0.5	0.2
Bialsche reagent (mL)	1	1	1	1	1	1	1	1
Boil for 12 min in water bath, then cool for 3 min with ice water
Pentanol (mL)	5	5	5	5	5	5	5	5
Shaken thoroughly and then centrifuged for 10 min at 1 000 r/min; took the pentanol phase for colorimetric analysis at a wavelength 569 nm