Protective effects of Asian green vegetables against oxidant induced cytotoxicity

Figure 4 Protective effects of vegetable extracts on ONOO-- and HOCl mediated cytotoxicity in HCT116 cells. Cells were pre-treated with each extract for 24 h to induce phase II detoxification enzymes prior to the addition of ONOO- or HOCl for 30 min. Cell viability was assayed using MTT and measurement of the solubilized formazan dye at Abs 595 nm. Experiments were conducted as described in Materials and methods and data are expressed as mean±SD (n = 6). ^bP<0.001 vs extracts to ONOO- or HOCl treatment alone.