Response of porcine hepatocytes in primary culture to plasma from severe viral hepatitis patients

Figure 6 Morphological changes of porcine hepatocytes cultured in 100% SVHP (A and B) and media containing 10% NCS (C and D). After 24 h of culture, hepatocytes in the100% SVHP group were detached from the dishes, vacuolization in the cytoplasm and deformities were more commonly observed. The membranes of cells became indistinct (A); after 48 h of culture, vacuoles were more and mainly concentrated around cell nuclei (B); after 24 h of culture, hepatocytes in the 10% NCS group constituted confluent monolayers with an intact morphology throughout the culture (C); after 48 h of culture, hepatocytes in the 10% NCS group constituted confluent monolayers with an intact morphology throughout the culture (D).