High affinity mouse-human chimeric Fab against Hepatitis B surface antigen

Figure 6 Expression profile of the chimeric Fab in different strains of E. coli. The chimeric Fab was expressed in soluble form in different E. coli strains in identical conditions. Levels of expression of the chimeric Fab were checked by Western blot in non-reducing conditions (A). Intensities of bands were measured densitometrically (B). Levels of expression were also checked by ELISA (C). Equal amount of the periplasmic extracts were allowed to bind to HBsAg coated on ELISA plate and bound chimeric Fab was detected. (XL1 = XL1-Blue, AD494 = AD494, COD+ = BL21 Codon Plus, TG1 = TG1, IDV = integrated density value).