High affinity mouse-human chimeric Fab against Hepatitis B surface antigen

doi:10.3748/wjg.v11.i48.7569

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Dec 28, 2005 (publication date) through Oct 2, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Dec 28, 2005; 11(48): 7569-7578
Published online Dec 28, 2005. doi: 10.3748/wjg.v11.i48.7569

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Figure 4 Binding properties of the anti-HBs chimeric Fab. 1 Solid phase ELISA was performed with different dilutions of the soluble chimeric Fab. The result of the competitive ELISA between the chimeric Fab and the mouse monoclonal 5S is shown in Figure (B). Different amounts of the chimeric Fab were allowed to compete with 1:80 dilution of the 5S-hybridoma culture supernatant for binding to HBsAg. Bound mouse monoclonal was detected with anti-mouse HRP and the % inhibition of binding was calculated.

Citation: Bose B, Khanna N, Acharya SK, Sinha S. High affinity mouse-human chimeric Fab against Hepatitis B surface antigen. World J Gastroenterol 2005; 11(48): 7569-7578
URL: https://www.wjgnet.com/1007-9327/full/v11/i48/7569.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i48.7569