Copyright
©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Dec 28, 2005; 11(48): 7569-7578
Published online Dec 28, 2005. doi: 10.3748/wjg.v11.i48.7569
Published online Dec 28, 2005. doi: 10.3748/wjg.v11.i48.7569
Figure 2 Phage ELISA to identify antigen binding clones.
After three rounds of biopanning, selected phage was rescued and antigen-binding clones were detected by phage ELISA. In this experiment 5S hybridoma supernatant and helper phage M13 KO7 were used as positive (+) and negative (–) control respectively. Clone 1 was used for soluble expression of the chimeric Fab.
- Citation: Bose B, Khanna N, Acharya SK, Sinha S. High affinity mouse-human chimeric Fab against Hepatitis B surface antigen. World J Gastroenterol 2005; 11(48): 7569-7578
- URL: https://www.wjgnet.com/1007-9327/full/v11/i48/7569.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i48.7569