Overexpression of NK2 inhibits liver regeneration after partial hepatectomy in mice

Figure 3 Expressions of RNAs and proteins of transgene and c-Met after PH. RNA expression of transgenic NK2 and c-Met, and c-Met protein levels and activity in livers from WT and NK2 transgenic mice after PH. Lane 1, WT mouse before PH; lane 2, WT mouse at 48 h after PH; lane 3, NK2 transgenic mouse before PH; lanes 4 and 5, dying NK2 transgenic mice at 48 h after PH; lanes 6 and 7, healthy NK2 transgenic mice at 48 h after PH. (A) Each RNA sample was subjected to Northern blot analysis. Expression levels of all transgenes were high at each time point and endogeneous c-Met expression was unaltered in each group. β-actin RNA expression was also examined as a control. (B) Each protein sample was subjected to IP and then IB with an anti c-Met antibody or an anti PY antibody. c-Met expression and the kinase activity were unaltered in each group.