Hepatocyte cytoskeleton during ischemia and reperfusion - influence of ANP-mediated p38 MAPK activation

Figure 8 Effect of p38 MAPK inhibition on phosphorylation status of Hsp27. Livers were perfused for 30 min in the absence (Co) or presence of 200 nmol/L ANP, which was added 20 min prior to 24-h ischemia (4 °C). In different experiments, livers were perfused for 20 min with SB203580 (2 μmol/L) or with a combination of SB203580 (2 μmol/L) and ANP (200 nmol/L). After ischemia, livers were snap-frozen and homogenized. Total Hsp27 was precipitated as described and the content of phosphorylated Hsp27 was determined by Western blotting and detection with anti-phospho-Hsp27 primary antibody. One representative Western blot is shown. In the graph hepatic content of phospho-Hsp27 is expressed as percent of control samples after 24-h ischemia as mean±SE of n = 4 experiments in each treatment group. ^aP<0.05 vs control after 24-h ischemia, ^bP<0.01 vs phospho-Hsp27 content in ANP pretreated livers.