Hepatocyte cytoskeleton during ischemia and reperfusion - influence of ANP-mediated p38 MAPK activation

Figure 7 Influence of pretreatment with ANP on phosphorylation status of Hsp27. After perfusion for 10 min with KH-buffer livers were preconditioned with ANP for 20 min or left untreated, followed by 24-h ischemia (4 °C) according to the study protocol. Samples were taken after 30-min perfusion and 24-h ischemia. Livers were snap-frozen and homogenized, followed by immunoprecipitation with an anti-Hsp27 antibody and Western blot analysis using an anti-phospho-Hsp27 antibody. One representative Western blot is shown. Content of phospho-Hsp27 in the liver homogenates is presented as 10-fold control after 30-min perfusion, using the respective control as reference value and expressed as mean±SE of n = 4 experiments in each treatment group. Statistical analysis of ANP pretreated samples: ^bP<0.001 vs phospho-Hsp27 in respective control (after 30-min perfusion or 24-h ischemia). Statistical analysis of untreated control samples: ^dP<0.01 vs phospho-Hsp27 in control after 30-min perfusion.