Hepatocyte cytoskeleton during ischemia and reperfusion - influence of ANP-mediated p38 MAPK activation

Figure 5 Changes in hepatic total Hsp27 during ischemia – influence of ANP pretreatment. Livers were perfused for 30 min in the presence or absence of ANP followed by 24-h ischemia at 4 °C in UW solution and reperfusion for 45 min. Afterwards, livers were snap-frozen at the indicated times, homogenized and analyzed via Western blot using an anti-Hsp27 antibody for detection of total Hsp27. One representative Western blot is shown for each experimental setting (untreated controls: (A), ANP pretreated livers: (B)). (A) Effect of IR on total Hsp27 in the liver. The graph shows percentage of values for untreated livers (Co) after 30-min perfusion (30' perf.) as means±SE of n = 4 experiments in each group. ^aP<0.05 vs untreated control after 30-min perfusion. (B) Influence of ANP preconditioning on total Hsp 27 after 24-h ischemia. Bars represent total Hsp27 as % Co after 24-h ischemia. Data are expressed as means±SE of n = 4 experiments in each treatment group. ^bP<0.01 vs control after 24-h ischemia.