Chloromycetin resistance of clinically isolated E coli is conversed by using EGS technique to repress the chloromycetin acetyl transferase

Figure 5 Electrophoresis of PCR amplification of EGS genes in the transformed bacteria after K plasmid and K3 plasmid introduced into 6 470. Lanes 1 and 10 Mmarker; Llane 2, blank control; lane 3, transformants cultured 2 d more; lane 4, original 6 470 strains; lanes 5, 6 and 7: 6 470 derivatives transformed with K3; lane 8: plasmid K3 (positive control); lane 9: K plasmid transformants (negative control).