Chloromycetin resistance of clinically isolated E coli is conversed by using EGS technique to repress the chloromycetin acetyl transferase

Figure 4 The map of plasmid extraction after the transformants were cultured for 2 d, K and TK can be detected in similar bands, while TK3 had no similar bands as K3, indicating the K3 plasmid was lost or had been degraded. M: Marker; lane K: control plasmid; K3; EGS plasmid; TK, 6 470 transformed with K plasmid; TK3, 6 470 transformed with K3; 6 470, original Cm-resistant 6 470.