Disrupted NF-κB activation after partial hepatectomy does not impair hepatocyte proliferation in rats

Figure 1 Effect of saline, PDTC, and PTX on NF-κB binding activity. A: Nuclear extracts were prepared from non-treated rats (control), from rats injected with saline, PDTC or PTX subjected or not to partial hepatectomy (PH). No NF-κB DNA binding activity was observed in the liver nuclear extracts of non-treated rats (controls) nor 1 h after injection of PDTC or PTX. Faint DNA binding was noticed 1 h after the injection of saline which mainly corresponds to NF-κB p50. Thirty minutes after PH, strong DNA binding was observed in saline-treated groups and two complexes were identified. Supershift experiments identified the upper complex in saline +PH as p65/p50. In the PTX group, a third complex was present 0.5 h after PH, which migrated faster than the dose obtained after saline; B: Detailed supershift experiments showed that this faster migrating complex in the PTX+PH group was completely supershifted with the p65 antibody. p52, c-rel and rel-B had no influence on the NF-κB DNA binding activity.