Small intestinal submucosa improves islet survival and function during in vitro culture

doi:10.3748/wjg.v11.i46.7378

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 14, 2005; 11(46): 7378-7383
Published online Dec 14, 2005. doi: 10.3748/wjg.v11.i46.7378

Table 2 Stimulated insulin secretion of rat islets cultured for 7 and 14 d during static incubation assay (n = 6, mean±SD)

Group	T (d)	Lowglucosesolution(2.7 mmol/L)	Highglucosesolution(16.7 mmol/L)	Highglucosesolution+50 μmol/L IBMX	SI¹
Standard culture	7	9.1±0.3	18.3±1.1	51.3±3.4	5.7±0.4
SIS culture	7	7.8±0.6	21.8±1.9	75.8±2.1^a	10.2±1.2^a
Standard culture	14	5.9±0.4	11.8±1.1	20.5±1.8	3.6±0.4
SIS culture	14	7.1±0.4	20.7±1.1^a	66.9±3.7^a	9.5±0.2^a

¹SI (stimulation index)=insulin secretion during incubation with 16.7 mmol/L high glucose solution+50 μmol/L IBMX/insulin secretion during incubation with 2.7 mmol/L low glucose solution.

^aP<0.05 vs standard culture.

Citation: Tian XH, Xue WJ, Ding XM, Pang XL, Teng Y, Tian PX, Feng XS. Small intestinal submucosa improves islet survival and function during in vitro culture. World J Gastroenterol 2005; 11(46): 7378-7383
URL: https://www.wjgnet.com/1007-9327/full/v11/i46/7378.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i46.7378